Helicobacter pylori Perturbs Iron Trafficking in the Epithelium to Grow on the Cell Surface
Figure 8
Hp colonization of the cell surface leads to transferrin transcytosis.
30 µg of biotin-albumin and 75 µg of biotin-transferrin were added to the basal chamber of uninfected or 2 day-infected polarized monolayers. The apical supernatant was sampled after a 24 hour incubation, and 10 µl of these samples separated by SDS-PAGE, blotted onto nitrocellulose, and the biotinylated albumin and transferrin were visualized with fluorescent streptavidin. The lane labeled “standard” is a 1∶250 dilution of the basal media containing biotin-albumin + biotin-transferrin. CagA* is the complemented ΔcagA mutant. Each band was quantified with the LI-COR Odyssey Scanner. Biotin-albumin amounts were used to normalize for loading. The graph depicts the average result from 6 experiments. p-values were obtained with a Wilcoxon signed rank test, using a hypothetical median of 1. N.S. indicates no statistical significance.
doi: https://doi.org/10.1371/journal.ppat.1002050.g008